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Chemical Synthesis of Shiga Toxin Subunit B Using a Next-Generation Traceless “Helping Hand” Solubilizing Tag

preprint
revised on 17.09.2019 and posted on 23.09.2019 by James Fulcher, Mark Petersen, riley giesler, Zachary Cruz, debra eckert, J. Nicholas Francis, eric kawamoto, Michael Jacobsen, Michael Kay
This manuscript describes the development and characterization of a second-generation "helping hand" solubilizing tag. This semipermanent tag aids solubility of peptides during purification and handling steps and is compatible with common native chemical ligation conditions. The utility of this tag is demonstrated by the synthesis of the Shiga toxin B subunit.

Funding

NIH AI150464

NIH AI120414

DoD W81XWH1810116

History

Email Address of Submitting Author

kay@biochem.utah.edu

Institution

University of Utah

Country

USA

ORCID For Submitting Author

0000-0003-3186-9684

Declaration of Conflict of Interest

MSK and DME are equity holders in Navigen Inc, which is developing D-peptide inhibitors.

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