Cambridge Open Engage home
What is Cambridge Open Engage?
How to Submit
Browse
About
News [opens in a new tab]

Chemical Synthesis of Shiga Toxin Subunit B Using a Next-Generation Traceless “Helping Hand” Solubilizing Tag

06 September 2019, Version 1
Working Paper
This content is a preprint and has not undergone peer review at the time of posting.

Abstract

This manuscript describes the development and characterization of a second-generation "helping hand" solubilizing tag. This semipermanent tag aids solubility of peptides during purification and handling steps and is compatible with common native chemical ligation conditions. The utility of this tag is demonstrated by the synthesis of the Shiga toxin B subunit.

Keywords

Native Chemical Ligation
Peptide synthesis
solubilizing groups
traceless tag
helping hand
Shiga toxin (Stx)
chemical Protein Synthesis

Supplementary materials

Title
Description
Actions
Title
HH2-Shiga ESI FINAL
Description
Actions

Comments

Comments are not moderated before they are posted, but they can be removed by the site moderators if they are found to be in contravention of our Commenting Policy [opens in a new tab] - please read this policy before you post. Comments should be used for scholarly discussion of the content in question. You can find more information about how to use the commenting feature here [opens in a new tab] .
This site is protected by reCAPTCHA and the Google Privacy Policy [opens in a new tab] and Terms of Service [opens in a new tab] apply.

Now Published

Chemical synthesis of Shiga toxin subunit B using a next-generation traceless “helping hand” solubilizing tag [opens in a new tab]
James M. Fulcher, Mark E. Petersen, Riley J. Giesler, Zachary S. Cruz, Debra M. Eckert, J. Nicholas Francis, Eric M. Kawamoto, Michael T. Jacobsen, Michael S. Kay journal article
Organic & Biomolecular Chemistry , Volume 17, Issue 48
Online publication date: 2019

Version History

Sep 06, 2019 Version 1

Metrics

3,387
822
0
Views
Downloads
Citations

License

CC logo
CC
BY logo
BY
NC logo
NC
ND logo
ND
The content is available under CC BY NC ND 4.0 [opens in a new tab]

DOI

10.26434/chemrxiv.9759314.v1
D O I: 10.26434/chemrxiv.9759314.v1 [opens in a new tab]

Funding

NIH AI150464
NIH AI120414
DoD W81XWH1810116

Author’s competing interest statement

MSK and DME are equity holders in Navigen Inc, which is developing D-peptide inhibitors.

Share