A Sustainable Approach to Cantherellus cibarius oil with Bioactive Properties Through Supercritical Fluid Extraction

23 June 2025, Version 1
This content is a preprint and has not undergone peer review at the time of posting.

Abstract

Cantherellus cibarius, sometimes known as the "boreal chanterelle," is a fungus species from the Cantharellaceae family. It is a member of the phylum Basidiomycota and one of the most sought edible mushrooms in the world. This study compared the use of supercritical fluid extraction (SFE), which is regarded as a mild and green extraction method, with the traditional solvent extraction to obtain Cantherellus cibarius oils. The optimized parameters of the SFE were obtained through a Box-Behnken design and the SFE approach provided oil yields that exceeded conventional extraction yields with ethyl acetate (10.0 % vs 8.1 %) while exhibiting better selectivity for the accumulation of lipophilic bioactives. Most importantly, the supercritically extracted oil showed significant radical scavenging activities against DPPH and ABTS, antioxidant and anti-inflammatory properties, as well as bactericidal effects against Bacillus cereus, Escherichia coli, and Salmonella typhi.

Keywords

supercritical fluid extraction
chanterelle oil
antioxidant
antimicrobial
anti-inflammatory

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