Comparative Analysis of the Chemical Composition and Cytotoxic Activity of Broccoli By-products as a Source of Bioactive Compounds

14 March 2025, Version 1
This content is a preprint and has not undergone peer review at the time of posting.

Abstract

Broccoli is one of the most widely consumed cruciferous vegetables worldwide; however, only about 30%—primarily the florets—are consumed, while the remaining parts are considered waste. In this study, we analyzed broccoli by-products from Mexican crops, specifically evaluating the ethanolic extracts of bagasse (BB) and residual leaves (BL) for their growth-inhibitory effects on PC-3, HeLa, and Hep3B cancer cell lines. Both BB and BL selectively inhibited cervical cancer cell proliferation by approximately 50%. A principal component analysis (PCA) of volatile compounds identified three main groups: fatty acids, methyl esters, sterols, and diterpenoids. Notably, sterols were the predominant components in the hexane fraction of broccoli bagasse (BB-H) (>90%), whereas fatty acids were the most abundant in the hexane fraction of broccoli leaves (BL-H) (>50%). Furthermore, two phytoalexins, caulilexin C (1) and 3-indoleacetonitrile (2), were isolated and characterized by NMR, exhibiting IC50 values of 23.43 and 33.65 μg/mL, respectively, against the HeLa cell line. Also, they showed desirable drug-like properties. These findings provide valuable insights into the chemical composition of broccoli by-products from Mexican crops, emphasizing their potential as a source of bioactive compounds with pharmaceutical and food applications.

Keywords

broccoli by-products
Brassica oleracea
cytotoxicity
phytoalexins

Supplementary materials

Title
Description
Actions
Title
SUPPORTING INFORMATION Comparative Analysis of the Chemical Composition and Cytotoxic Activity of Broccoli By-products as a Source of Bioactive Compounds
Description
Spectra and tables of the chemical charactariztion of: Comparative Analysis of the Chemical Composition and Cytotoxic Activity of Broccoli By-products as a Source of Bioactive Compounds
Actions

Comments

Comments are not moderated before they are posted, but they can be removed by the site moderators if they are found to be in contravention of our Commenting Policy [opens in a new tab] - please read this policy before you post. Comments should be used for scholarly discussion of the content in question. You can find more information about how to use the commenting feature here [opens in a new tab] .
This site is protected by reCAPTCHA and the Google Privacy Policy [opens in a new tab] and Terms of Service [opens in a new tab] apply.