Cambridge Open Engage home
What is Cambridge Open Engage?
How to Submit
Browse
About
News [opens in a new tab]

Structural Heterogeneity of the Humanised IgGk NIST Monoclonal Antibody Probed by Tandem-Trapped Ion Mobility Spectrometry and Mobility-Resolved Collision-induced Unfolding.

02 July 2024, Version 2
Working Paper
This content is a preprint and has not undergone peer review at the time of posting.

Abstract

Investigating the structural heterogeneity of monoclonal antibodies is crucial to achieving optimal therapeutic outcomes. We show that tandem-trapped ion mobility spectrometry enables collision-induced unfolding measurements of antibody subpopulations. Our data suggest that such non-ensemble measurements will improve the structural characterisation of complex biotherapeutics such as multivalent antibodies.

Keywords

monoclonal antibodies
structural heterogeneity
ion mobility spectrometry/mass spectrometry
Tandem-TIMS
collision-induced unfolding

Supplementary materials

Title
Description
Actions
Title
Supporting Information
Description
mass spectra; comparison of cross-sections by Tandem-TIMS and DTIMS; ensemble CIU spectra; Structure Relaxation Approximation calculation.
Actions

Comments

Comments are not moderated before they are posted, but they can be removed by the site moderators if they are found to be in contravention of our Commenting Policy [opens in a new tab] - please read this policy before you post. Comments should be used for scholarly discussion of the content in question. You can find more information about how to use the commenting feature here [opens in a new tab] .
This site is protected by reCAPTCHA and the Google Privacy Policy [opens in a new tab] and Terms of Service [opens in a new tab] apply.

Version History

Jul 02, 2024 Version 2

Version Notes

add Funding agency

Metrics

426
194
0
Views
Downloads
Citations

License

CC logo
CC
BY logo
BY
NC logo
NC
ND logo
ND
The content is available under CC BY NC ND 4.0 [opens in a new tab]

DOI

10.26434/chemrxiv-2024-7hj35-v2
D O I: 10.26434/chemrxiv-2024-7hj35-v2 [opens in a new tab]

Funding

National Science Foundation
CHE 2305173
National Institutes of Health
R01GM135682

Author’s competing interest statement

S.P. and E.M.P. are employees at Bruker Corporation, which sells timsTOF instruments.

Ethics

The author(s) have declared ethics committee/IRB approval is not relevant to this content

Share