Abstract
Investigating the structural heterogeneity of monoclonal antibodies is crucial to achieving optimal therapeutic outcomes. We show that tandem-trapped ion mobility spectrometry enables collision-induced unfolding measurements of antibody subpopulations. Our data suggest that such non-ensemble measurements will improve the structural characterisation of complex biotherapeutics such as multivalent antibodies.
Supplementary materials
Title
Supporting Information
Description
mass spectra; comparison of cross-sections by Tandem-TIMS and DTIMS; ensemble CIU spectra; Structure Relaxation Approximation calculation.
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