Abstract
We report two related experiments to introduce column chromatography in the context of a second-year organic chemistry lab course or an upper-division advanced organic chemistry lab course. Both experiments involve functionalization of guaiazulene via electrophilic aromatic substitution, and the highly colored starting material and products serve as clear visual indicators of reaction progress and of the purification. The longer version of the experiment scaffolds the chromatography process by guiding students to develop conditions to purify their own unique compound. The products are amenable to a wide variety of characterization techniques, including UV-visible absorbance spectroscopy and 2D NMR spectroscopy. These experiments are highly flexible and have been implemented in a second-year course as an activity taking a single lab period and in an upper-division course as an activity taking three full lab periods.
Supplementary materials
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Notes for Instructors and Characterization Data (pdf)
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Pdf version of instructor notes and characterization data.
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Notes for Instructors and Characterization Data (docx)
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Editable docx version of instructor notes and characterization data.
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Student Lab Procedures (pdf)
Description
Pdf version of the student lab procedure handouts.
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Student Lab Procedures (docx)
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Editable docx version of the student lab procedure handouts.
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MNova Files of Spectroscopic Data
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Zip folder containing MNova files with GC/MS, IR, and NMR data for each reactant/product.
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Absorbance Spectroscopy Data
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UV/visible absorbance spectroscopy data for each reactant/product.
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Supplemental Video
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Video showing process of column chromatography for purifying compound 3 (video is sped up to 33x speed).
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