Imaging-based profiling for elucidation of antibacterial mechanisms of action

15 May 2024, Version 1
This content is a preprint and has not undergone peer review at the time of posting.


In this review, we aim to summarize experimental data and approaches to identifying cellular targets or mechanisms of action of antibacterials based on imaging techniques. Imaging-based profiling methods such as bacterial cytological profiling, dynamic bacterial morphology imaging and others have become a useful research tool for mechanistic studies of new antibiotics as well as combinations with conventional ones and other therapeutic options. The main methodological, experimental details and obtained results are summarized and discussed. The review covers the literature up to Feb 2024.


bacterial phenotype
bacterial imaging
mechanism of action
fluorescent microscopy


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