Database Assembly for Peptide Mass Fingerprinting

24 August 2023, Version 2
This content is a preprint and has not undergone peer review at the time of posting.

Abstract

The idea of peptide mass fingerprinting (PMF) was first introduced in 1989, when protein research was facing a serious issue with automated Edman-degradation taking nearly one hour per reaction cycle. There was a dire need for more streamlined and fast ways to analyse proteins and peptides. Since the first steps towards such rapid analysis were made using FAB-MS and a relatively simple computer program called FRAGFIT, followed by the consecutive introduction of peptide mass fingerprinting as a simple yet effective tool to quickly identify frequently observed proteins in electrophoresis gels, PMF has developed to become the foundation of modern proteomics research and paved the way for many more high-throughput techniques, including shotgun proteomics and other bottom-up workflows.

Keywords

Peptide Mass Fingerprinting
MASCOT
Proteomics

Comments

Comments are not moderated before they are posted, but they can be removed by the site moderators if they are found to be in contravention of our Commenting Policy [opens in a new tab] - please read this policy before you post. Comments should be used for scholarly discussion of the content in question. You can find more information about how to use the commenting feature here [opens in a new tab] .
This site is protected by reCAPTCHA and the Google Privacy Policy [opens in a new tab] and Terms of Service [opens in a new tab] apply.