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Abstract
Resin 3D printing is attractive for rapid fabrication of microscale cell culture devices, but common resin materials are unstable and cytotoxic under culture conditions. Strategies such as leaching or over-curing are insufficient to protect sensitive primary cells such as white blood cells. Here, we evaluated the effectiveness of using parylene-C coating of commercially available clear resins to prevent cytotoxic leaching, degradation of microfluidic devices, and absorption of small molecules. We found that parylene-C significantly improved both the cytocompatibility with primary murine white blood cells and the material integrity of prints, while maintaining the favorable optical qualities held by clear resins.
Supplementary materials
Title
Electronic Supplementary Materials
Description
Supplementary materials including the following:
• Design Files
▪ Access Link to 3D printed files used in the main text (Figure 2a-c)
• Figures
▪ Supplemental Figure S1: Gating strategy and single stain controls for flow
cytometry
▪ Supplemental Figure S2: Combined transparency data for four commercial
resins, uncoated and coated.
▪ Supplemental Figure S3: Combined autofluorescence data for four
commercial resins, uncoated and coated.
• Protocols
▪ Supplemental Protocol S1: 3D printing parts.
▪ Supplemental Protocol S2: Parylene coating procedure with the SCS
Labcoater 2.
▪ Supplemental Protocol S3: Testing cell viability of murine splenocytes.
▪ Supplemental Protocol S4: Testing erosion of chips after repeated uses.
▪ Supplemental Protocol S5: Microscopy for brightfield and fluorescent
images.
▪ Supplemental Protocol S6: Measuring surface roughness.
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