Development and analytical validation of a method for the determination of lead, cadmium, mercury and their isotopes in milk, honey, meat, fish and eggs by icp-ms

recent decades, industrial and urban development has been accompanied by real health problems linked to environmental pollution and the contamination of foodstuffs of animal origin by heavy metals such as lead, cadmium and mercury which are toxic to the immune, hematological, renal and neurological system with a risk of developing cancer in the consumer of contaminated food. The aim of this study was to develop and validate an analytical method to determine the metals Cadmium (111Cd, 114Cd), Lead (208Pb) and Mercury (202Hg) in milk, honey, meat, fish and eggs using inductively coupled plasma mass spectrometry (ICP-MS). For sample preparation, microwave oven digestion with nitric acid and hydrogen peroxide is adopted. The method showed linearity, accuracy and limits of quantification and detection acceptable for LABGTA45 protocol validation of analytical methods in the area of food analysis. Therefore, the method using ICP-MS was developed and validated to determine metals concentrations in food matrixes and the proposed method could be applied in routine analytical laboratory.