Abstract
Fluorescent ligands for G-protein coupled receptors (GPCRs) are valuable tools for studying the expression, pharmacology and modulation of these therapeutically important proteins in living cells. Here we report a photocrosslinking fluorescent chemical probe for Formyl peptide receptor 1 (FPR1), a critical component of the innate immune response to bacterial infection and a promising target in inflammatory diseases. We demonstrate that the probe binds and covalently crosslinks to FPR1 with high specificity at nanomolar concentrations in living cells and is a useful tool for visualisation and characterisation of this receptor.
Supplementary materials
Title
Field et al. Supplementary Methods and Results
Description
Experimental methods associated with the paper and additional results (Supplementary Figures S1-S7)
Actions