Working Paper
Authors
- Navid Shakiba Universität Hamburg ,
- Henri Lösel Universität Hamburg ,
- Soeren Wenk Universität Hamburg ,
- René Bachmann Landeslabor ,
- Stephan Seifert Universität Hamburg ,
- Markus Fischer Universität Hamburg ,
- Thomas Hackl
Universität Hamburg
Abstract
Storage is a critical step in post-harvest processing of hazelnuts since it can lead to mold, rancidity and off-flavor. To comprehensively investigate the effects of hazelnut storage, samples were stored under three short-term and two long-term conditions and the polar metabolome was analyzed by 1H NMR spectroscopy at different timepoints. Large differences in the polar metabolome were observed between the investigated storage conditions. Classification between samples pre- and post-storage showed that samples stored under harsh conditions for a short period and under standard conditions long-term were almost always classified correctly. Boruta, a feature selection algorithm, was used to select metabolites important for classification. Increases in hexanoic, octanoic, and nonanoic acid, all products of lipid oxidation, were found across all conditions and an increase in formic acid in most. Harsh short-term storage resulted in increases in fumaric and lactic acid, glucose, fructose and choline and a decrease in acetic acid.
Content

Supplementary material

Monitoring metabolic changes during storage of hazelnuts (Corylus avellana L.) by H NMR spectroscopy
Bins selected by Boruta as well as further classification and regression results are presented in the Supplementary Material.