Organic Chemistry

Structure and Function of NzeB, a Versatile C–C and C–N Bond Forming Diketopiperazine Dimerase


This report details the discovery and characterization of a versatile bacterial cytochrome P450, NzeB, which catalyzes the dimerization of diketopiperazines via enzymatic C–H functionalization. This includes the first high-resolution crystal structure of a diketopiperazine dimerase, which along with active site via mutagenesis and quantum mechanical calculations, provides insight into the selectivity and mechanism of these enzymes.


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Supplementary material

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NzeB SI 2020 05 14 Combined