Structure and Function of NzeB, a Versatile C–C and C–N Bond Forming Diketopiperazine Dimerase


This report details the discovery and characterization of a versatile bacterial cytochrome P450, NzeB, which catalyzes the dimerization of diketopiperazines via enzymatic C–H functionalization. This includes the first high-resolution crystal structure of a diketopiperazine dimerase, which along with active site via mutagenesis and quantum mechanical calculations, provides insight into the selectivity and mechanism of these enzymes.


Supplementary material

NzeB SI 2020 05 14 Combined