Structure and Function of NzeB, a Versatile C–C and C–N Bond Forming Diketopiperazine Dimerase

15 May 2020, Version 1
This content is a preprint and has not undergone peer review at the time of posting.

Abstract

This report details the discovery and characterization of a versatile bacterial cytochrome P450, NzeB, which catalyzes the dimerization of diketopiperazines via enzymatic C–H functionalization. This includes the first high-resolution crystal structure of a diketopiperazine dimerase, which along with active site via mutagenesis and quantum mechanical calculations, provides insight into the selectivity and mechanism of these enzymes.

Keywords

cytochrome P450
diketopiperazine
diketopiperazine dimers
Natural products
biosynthesis
C–H functionalization

Supplementary materials

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Description
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Title
NzeB SI 2020 05 14 Combined
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