Probing Cellular Outcomes Using Heterobivalent Constructs

22 January 2019, Version 1
This content is a preprint and has not undergone peer review at the time of posting.


Conjugation techniques are central to improving intracellular delivery of bioactive small molecules. However, tracking and assessing the overall biological outcome of these constructs remains poorly understood. We addressed this issue by having developed a focused library of heterobivalent constructs based on Rho kinase inhibitors to probe various scenarios. By comparing induction of a phenotype of interest vs. cell viability vs. cellular uptake, we demonstrate that such conjugates indeed lead to divergent cellular outcomes.


chemical probes
fluorescent probes
bile acids
phenotypic characterization
cellular uptake

Supplementary materials

Bhadoria et al SI


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