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Structure and Function of NzeB, a Versatile C–C and C–N Bond Forming Diketopiperazine Dimerase

preprint
submitted on 14.05.2020, 20:37 and posted on 15.05.2020, 09:10 by Vikram V. Shende, Yogan Khatri, Sean A. Newmister, Jacob N. Sanders, Petra Lindovska, Fengan Yu, Tyler J. Doyon, Justin Kim, Mohammad Movassaghi, Kendall N Houk, David H. Sherman
This report details the discovery and characterization of a versatile bacterial cytochrome P450, NzeB, which catalyzes the dimerization of diketopiperazines via enzymatic C–H functionalization. This includes the first high-resolution crystal structure of a diketopiperazine dimerase, which along with active site via mutagenesis and quantum mechanical calculations, provides insight into the selectivity and mechanism of these enzymes.

Funding

CHE-1700982

GM118101

GM089732

GM122218

ACI-1548562

History

Email Address of Submitting Author

davidhs@umich.edu

Institution

University of Michigan

Country

United States

ORCID For Submitting Author

0000-0001-8334-3647

Declaration of Conflict of Interest

The authors declare no competing financial interest.

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