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Structure and Function of NzeB, a Versatile C–C and C–N Bond Forming Diketopiperazine Dimerase

preprint
submitted on 14.05.2020 and posted on 15.05.2020 by Vikram V. Shende, Yogan Khatri, Sean A. Newmister, Jacob N. Sanders, Petra Lindovska, Fengan Yu, Tyler J. Doyon, Justin Kim, Mohammad Movassaghi, Kendall N Houk, David H. Sherman
This report details the discovery and characterization of a versatile bacterial cytochrome P450, NzeB, which catalyzes the dimerization of diketopiperazines via enzymatic C–H functionalization. This includes the first high-resolution crystal structure of a diketopiperazine dimerase, which along with active site via mutagenesis and quantum mechanical calculations, provides insight into the selectivity and mechanism of these enzymes.

Funding

CHE-1700982

GM118101

GM089732

GM122218

ACI-1548562

History

Email Address of Submitting Author

davidhs@umich.edu

Institution

University of Michigan

Country

United States

ORCID For Submitting Author

0000-0001-8334-3647

Declaration of Conflict of Interest

The authors declare no competing financial interest.

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