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Influence of a Single Ether Bond on Assembly, Orientation, and Miscibility of Phosphocholine Lipids at the Air-Water Interface

preprint
revised on 15.12.2020, 07:51 and posted on 16.12.2020, 12:05 by Matthias Hoffmann, Simon Drescher, Christian Schwieger, Dariush Hinderberger
How does a small change in the structure of a phospholipid affect its supramolecular assembly? In aqueous suspensions, the substitution of one ester linkage in DPPC (1,2-dipalmitoyl-sn-glycero-3-phosphocholine) by an ether linkage alters its phase behaviour completely. To unravel the effect of replacing a phospholipid’s ester linkage by an ether linkage in lipid monolayers, we characterized pure monolayers of the model lipid DPPC and its sn-2 ether analogue PHPC (1-palmitoyl-2-O-hexadecyl-sn-glycero-3-phosphocholine) as well as mixtures of both by measurements of surface pressure – molecular area (π–A_mol) isotherms. In addition, we used infrared reflection absorption spectroscopy (IRRAS) and epifluorescence microscopy to study lipid condensation, lipid chain orientation, headgroup hydration, and lipid miscibility in all samples.

Funding

Fonds der Chemischen Industrie (FCI)

History

Email Address of Submitting Author

dariush.hinderberger@chemie.uni-halle.de

Institution

Martin-Luther-Universität Halle-WIttenberg, Institute of Chemistry

Country

Germany

ORCID For Submitting Author

0000-0002-6066-7099

Declaration of Conflict of Interest

no conflicts

Version Notes

Version 1, 12.12..2020

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