Influence of a Single Ether Bond on Assembly, Orientation, and Miscibility of Phosphocholine Lipids at the Air-Water Interface

15 December 2020, Version 1
This content is a preprint and has not undergone peer review at the time of posting.

Abstract

How does a small change in the structure of a phospholipid affect its supramolecular assembly? In aqueous suspensions, the substitution of one ester linkage in DPPC (1,2-dipalmitoyl-sn-glycero-3-phosphocholine) by an ether linkage alters its phase behaviour completely. To unravel the effect of replacing a phospholipid’s ester linkage by an ether linkage in lipid monolayers, we characterized pure monolayers of the model lipid DPPC and its sn-2 ether analogue PHPC (1-palmitoyl-2-O-hexadecyl-sn-glycero-3-phosphocholine) as well as mixtures of both by measurements of surface pressure – molecular area (π–A_mol) isotherms. In addition, we used infrared reflection absorption spectroscopy (IRRAS) and epifluorescence microscopy to study lipid condensation, lipid chain orientation, headgroup hydration, and lipid miscibility in all samples.

Keywords

lipids
self-assembly
ether lipids
archaeal components
DPPc
PHPC
hydration
IRRAS

Supplementary materials

Title
Description
Actions
Title
TOC PHPC
Description
Actions
Title
PHPC 20201211 final SI
Description
Actions
Title
PHPC 20201211 final ChemRxiv
Description
Actions

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