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In Vivo Assembly of Artificial Metalloenzymes and Application in Whole‐Cell Biocatalysis

preprint
submitted on 15.06.2020 and posted on 17.06.2020 by Shreyans Chordia, Siddarth Narasimhan, Alessandra Lucini Paioni, Marc Baldus, Gerard Roelfes
Artificial metalloenzymes (ArMs), which are hybrids of catalytically active transition metal complexes and proteins, have emerged as promising approach to the creation of biocatalysts for reactions that have no equivalent in nature. Here we report the assembly and application in catalysis of ArMs in the cytoplasm of E. coli cells based on the Lactococcal multidrug resistance regulator (LmrR) and an exogeneously added copper(II)‐phenanthroline (Cu(II)‐phen) complex. The ArMs are spontaneously assembled by addition of Cu(II)‐phen to E. coli cells that express LmrR and it is shown that the ArM containing whole cells are active in the catalysis of the enantioselective vinylogous Friedel‐Crafts alkylation of indoles. The ArM assembly in E. coli is further supported by a combination of cell‐ fractionation and inhibitor experiments and confirmed by in‐cell solid‐state NMR. A mutagenesis study showed that the same trends in catalytic activity and enantioselectivity in response to mutations of LmrR were observed for the ArM containing whole cells and the isolated ArMs. This made it possible to perform a directed evolution study using ArMs in whole cells, which gave rise to a mutant, LmrR_A92E_M8D that showed increased activity and enantioselectivity in the catalyzed vinylogous Friedel‐Crafts alkylation of a variety of indoles. The unique aspect of this whole‐cell ArM system is that no engineering of the microbial host, the protein scaffold or the cofactor is required to achieve ArM assembly and catalysis. This makes this system attractive for applications in whole cell biocatalysis and directed evolution, as demonstrated here. Moreover, our findings represent important step forward towards achieving the challenging goal of a hybrid metabolism by integrating artificial metalloenzymes in biosynthetic pathways.

Funding

Nederlandse Organisatie voor Wetenschappelijk Onderzoek, grant numbers: 700.26.121; 700.10.443;724.013.003

Ministerie van Onderwijs, Cultuur en Wetenschappen, Gravitation program no.024.001.035

NMARRS, project number 022.005.029

History

Email Address of Submitting Author

j.g.roelfes@rug.nl

Institution

University of Groningen, Stratingh Institute for Chemistry

Country

the Netherlands

ORCID For Submitting Author

0000-0002-0364-9564

Declaration of Conflict of Interest

no conflict of interest

Version Notes

1st version

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