ChemRxiv
These are preliminary reports that have not been peer-reviewed. They should not be regarded as conclusive, guide clinical practice/health-related behavior, or be reported in news media as established information. For more information, please see our FAQs.
Anthocyanins from the Red Juvenile Leaves of Loropetalum Chinense var. rubrum (Chinese Fringe Flower) Identification and pH Sensing Behaviors.pdf (607.4 kB)

Anthocyanins from the Red Juvenile Leaves of Loropetalum Chinense var. rubrum (Chinese Fringe Flower) Identification and pH Sensing Behaviors

preprint
submitted on 27.10.2020, 16:05 and posted on 29.10.2020, 07:06 by Chengkun He, Yufei Jiang, Hongbao Fang, Yujuan Zhang, Zijian Guo

In this study, anthocyanins were extracted from the frozen juvenile leaves of Loropetalum Chinense var. rubrum with high efficiency using ethanol containing 0.1% HCl as extractant. After being purified with AB-8 resin, this extract was investigated with HPLC-DAD-MS/MS determination, and six anthocyanins were identified according to chromatogram, MS data and ratios of absorbance at 400-460 nm to that at absorption maximum (~520 nm), A400-460/A520. Besides cyanidin 3-O-glucoside, peonidin 3-O-glucoside, malvidin 3-O-glucoside, the most abundant malvidin 3,5-O-diglucoside was confirmed through preparative HPLC separation and 1 H NMR determination. However, two delphinidin- and petunidin-derived anthocyanins should be further clarified whether they are 3,5-O-diglucoside or 5-O-glucobioside. In addition, a novel anthocyanin, 2H-pyran[5,6]malvidin 3-O-glucobioside was also proposed yet need more data to confirm. Moreover, malvidin 3,5-O-diglucoside showed excellent pH sensing ability, displaying distinct color change from pink (pH 1.5) to colorlessness (pH 5.5), to blue (pH 6.8) and finally green (pH 8.8 to pH 10.5). All these should be helpful to evaluate this plant as natural pigment resource.

History

Email Address of Submitting Author

mike_chengkunhe@qq.com

Institution

Nanjing Foreign Language School

Country

China

ORCID For Submitting Author

0000-0002-7127-0097

Declaration of Conflict of Interest

There is no conflict of interests.

Exports

ChemRxiv

Categories

Exports