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manuscript for SARS-CoV2 vaccine 20200512.pdf (1020.89 kB)

A Translatable Subunit Nanovaccine for COVID-19

preprint
submitted on 14.05.2020, 08:56 and posted on 15.05.2020, 09:59 by Lixin Liu, Zhijia Liu, Haolin Chen, Hong Liu, Qiang Gao, Feng Cong, Guangxia Gao, Yongming Chen
In order to combat severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic in the world, we formulate S1 subunit of the virus with two types of adjuvants, amphiphilic adjuvant monophosphoryl lipid A (MPLA) for Toll-like receptor 4 (TLR4) and CpG ODN for TLR9, into cationic multifunctional liposomes to produce a potent, safer, and translatable nanovaccine. The results show that the nanovaccine can efficiently elicit humoral immune response in mice. The sera from the vaccinated mice significantly inhibit SARS-CoV-2 to infect Vero cells. Moreover, relatively to the free S1 with traditional Alum adjuvant, the nanovaccine can elicit strong T cell immunity by activating both CD4+ and CD8+ cells, which may play critical roles in eliminating viral load in patients. Most importantly, the nanovaccine can elicit strong IgA antibody, providing potential mucosal protection to host. Altogether, this study offers a translatable design for a potent subunit SARS-CoV-2 nanovaccine.

Funding

the National Natural Science Foundation of China (51820105004)

the Guangdong Innovative and Entrepreneurial Research Team Program (2013S086)

History

Email Address of Submitting Author

chenym35@mail.sysu.edu.cn

Institution

Sun Yat-sen University

Country

China

ORCID For Submitting Author

0000-0003-2843-5543

Declaration of Conflict of Interest

We declare no conflict of interest

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