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Peptide Identification and Quantification by Gas Phase Fractionation Enables Proteomics Without Liquid Chromatography

preprint
submitted on 25.05.2020, 18:29 and posted on 27.05.2020, 08:43 by Jesse Meyer, Natalie M. Niemi, David J. Pagliarini, Joshua J. Coon

Liquid chromatography mass spectrometry (LC-MS) delivers sensitive peptide analysis for proteomics, but the methodology requires extensive analysis time, hampering throughput. Here, we demonstrate that flow injection analysis data-independent acquisition (FIA-DIA), using gas-phase peptide separation instead of LC, offers extremely fast proteome analysis. Incorporating ion mobility with FIA-DIA, we demonstrate the targeted quantification of over 500 proteins within minutes of MS data collection (~3.5 proteins/second). We show the utility of this technology to perform a complex multifactorial proteome study of interactions between nutrients, genotype, and mitochondrial toxins in a collection of cultured human cells. More than 45,000 quantitative protein measurements from 132 samples were achieved in only 4.4 hours of MS data collection. Enabling fast, unbiased proteome quantification without LC, FIA-DIA offers a new approach to boosting throughput critical to drug and biomarker discovery studies that require analysis of thousands of proteomes.

History

Email Address of Submitting Author

jessegmeyer@gmail.com

Institution

University of Wisconsin, Madison

Country

United States

ORCID For Submitting Author

0000-0003-2753-3926

Declaration of Conflict of Interest

JJC is a consultant for ThermoFisher

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