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COVID-19: Attacks the 1-Beta Chain of Hemoglobin and Captures the Porphyrin to Inhibit Human Heme Metabolism

preprint
revised on 22.03.2020 and posted on 23.03.2020 by liu wenzhong, Li hualan

The novel coronavirus pneumonia (COVID-19) is an infectious acute respiratory infection caused by the novel coronavirus. The virus is a positive-strand RNA virus with high homology to bat coronavirus. In this study, conserved domain analysis, homology modeling, and molecular docking were used to compare the biological roles of certain proteins of the novel coronavirus. The results showed the ORF8 and surface glycoprotein could bind to the porphyrin, respectively. At the same time, orf1ab, ORF10, and ORF3a proteins could coordinate attack the heme on the 1-beta chain of hemoglobin to dissociate the iron to form the porphyrin. The attack will cause less and less hemoglobin that can carry oxygen and carbon dioxide. The lung cells have extremely intense poisoning and inflammatory due to the inability to exchange carbon dioxide and oxygen frequently, which eventually results in ground-glass-like lung images. The mechanism also interfered with the normal heme anabolic pathway of the human body, is expected to result in human disease. According to the validation analysis of these finds, chloroquine could prevent orf1ab, ORF3a, and ORF10 to attack the heme to form the porphyrin, and inhibit the binding of ORF8 and surface glycoproteins to porphyrins to a certain extent, effectively relieve the symptoms of respiratory distress. Favipiravir could inhibit the envelope protein and ORF7a protein bind to porphyrin, prevent the virus from entering host cells, and catching free porphyrins. Because the novel coronavirus is dependent on porphyrins, it may originate from an ancient virus. Therefore, this research is of high value to contemporary biological experiments, disease prevention, and clinical treatment.

History

Email Address of Submitting Author

liuwz@suse.edu.cn

Institution

Sichuan University of Science & Engineering

Country

China

ORCID For Submitting Author

0000-0003-3670-3915

Declaration of Conflict of Interest

No

Version Notes

1.Rename Title 2.Abstract(added): (1)Viral non-structural protein attacks the heme on the beta chain of the hemoglobin (2)reason of respiratory distress and ground-glass-like lung images (3)Validation for the effect of Favipiravir 3.Method (added): Protein docking... 4.Results(added): (1)Viral non-structural protein attacks the heme on the beta chain of the hemoglobin (2)Validation for the effect of Favipiravir 5.Discussion (added): (1)The novel coronavirus originated from an ancient virus (2)Higher permeability of porphyrins into cell membranes leads to high infections (3)Higher hemoglobin caused higher morbidity (4)Inhibiting the heme anabolic pathway and causing the disease (5)The complexity of individual immunity 6.Conclusions(added): (1)Viral non-structural protein attacks the heme on the beta chain of the hemoglobin (2)reason of respiratory distress and ground-glass-like lung images (3)Validation for the effect of Favipiravir

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