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Abstract
A urease–BTB/silicalite optical biosensor was developed for real-time urea detection. The silicalite matrix provided a stable platform for urease immobilization, enabling reliable enzymatic activity and a measurable reflectance response. The biosensor demonstrated high sensitivity, reproducibility, and selectivity, with strong agreement to standard methods. Successful detection in environmental water samples highlights its applicability for urea monitoring in complex matrices.
Supplementary materials
Title
High-Performance Urease-BTB/Silicalite Optical Biosensor for Real-Time Urea Detection
Description
The authors declare that there are no competing interests associated with this manuscript. None of the authors have any financial, personal, or professional relationships that could be construed to influence the content or outcome of this work. The research was conducted independently, without involvement from organizations that could gain or lose financially through its publication. All authors have reviewed and approved the final version of the manuscript and affirm that there are no conflicts of interest to disclose
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