Pure chitosan microfluidic spinning affords modular core-sheath fibers and hand-crafted 3D scaffolds with enhanced fibroblasts compatibility

Microfluidic spun hydrogel fibers are appealing for tissue engineering and cell transplantation applications because they can feature hierarchical organization, have the ability to be woven or self-assembled into macro-objects, and can be easily functionalized or used for cell or chemical encapsulation. They have been developed as templates for reconstructing fiber-shaped tissues and mimic blood vessels, muscle fibers or neural networks in vivo. Alginates are the overwhelmingly employed materials to fabricate continuous hydrogel microfiber substrates, because of the simplicity of their processing. They, however, suffer from poor cell adherence and weak cell-matrix interactions. Alginates also need crosslinking, and thus easily lose shape and leach ions in the physiological environment. To overcome these challenges, we are reporting herein the first synthesis of pure chitosan by microfluidics, avoiding the use of any crosslinking agent. These fibers have remarkable cell viability of 85%, in absence of any special coating, and a comparatively higher mechanical strength than known alginate ones. Our system also demonstrated the synthesis of chitin nanocrystals/chitosan composite microfibers with remarkable grooved surface. Chitosan is a natural biopolymer obtained from the deacetylation of chitin, which is found in crustacean shells, insect cuticles, and fungi, and thus represents a sustainable source. Therefore, working with chitosan-derived materials helps us to address SDGs 6 and 14. The fibers of pure chitosan and chitosan composite exhibit outstanding processability and can be woven into a variety of structures. These microstructured chitosan fibers have the potential to be used as templates to create fiber-shaped tissues or to develop into live building blocks for the assembly of very complex artificial tissues.