Detection and resolution of sub-millimolar concentration amino acids on a benchtop NMR spectrometer by nh-PHIP hyperpolarization

04 June 2025, Version 1
This content is a preprint and has not undergone peer review at the time of posting.

Abstract

Considerable purchase and maintenance costs of high-field NMR instruments restrict their applications in bio- and chemical laboratories. Benchtop spectrometers offer an affordable alternative, but are limited in their sensitivity and resolution. Herein, we demonstrate how parahydrogen based nh-PHIP hyperpolarization can be used to circumvent these hindrances, allowing to detect and resolve a mixture of dilute amino acids on an 80 MHz spectrometer.

Keywords

NMR
nh-PHIP
hyperpolarization
low-field
amino acids

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