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A Perspective on Cysteine-Reactive Activity-based Probes

03 June 2025, Version 1
Review
This content is a preprint and has not undergone peer review at the time of posting.

Abstract

Activity-based protein profiling (ABPP) is a powerful method to interrogate protein function, identify protein targets and mechanisms-of-action of chemical probes as well as small molecule drugs. Our perspective provides an overview on a selec-tion of ABPP techniques and explores their potential, limitations as well as opportunities.

Keywords

Cysteine-reactive chemotypes
Activity-Based Protein Profiling
Chemoproteomics
Chemical Probe Development

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Version History

Jun 03, 2025 Version 1

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The content is available under CC BY NC ND 4.0 [opens in a new tab]

DOI

10.26434/chemrxiv-2025-8cv0p
D O I: 10.26434/chemrxiv-2025-8cv0p [opens in a new tab]

Funding

Fonds der Chemischen Industrie
Liebig Fellowship
Fonds der Chemischen Industrie
PhD Fellowship
Deutsche Forschungsgemeinschaft
SPP 2306 (KO 5903/2-1, No. 461576077)

Author’s competing interest statement

The author(s) have declared they have no conflict of interest with regard to this content

Ethics

The author(s) have declared ethics committee/IRB approval is not relevant to this content

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