Variable pathlength cell for internal data validation in computer vision

Computer vision has emerged as a fast and cost-effective method for reaction monitoring and determination of analytes. However, one of the drawbacks of computer vision in analytical chemistry is data reliability, particularly in data acquired from in-situ and real-time analyses, inhibiting qualitative as well as quantitative interpretation. This emphasises the need for an effective validation method for data acquired using computer vision. Here, we report a simple yet effective variable pathlength cell design that can help data validation in computer vision by exploiting the linear pathlength-absorbance relationship of the Beer-Lambert law. The performance of this novel variable pathlength cell is tested using a wide range of concentrations of an analyte. This variable pathlength cell design is versatile and can be fabricated using various methodologies and materials. This design, combined with computer vision, is compatible with flow chemistry and holds great potential to be integrated into automatic, inline quantitative analysis of reactions and analytical chemistry.