A Universal Oxygen Scavenger for Oxidase-based Biosensors

EOxidase-based electrochemical biosensors are widely deployed for point-of-use applications, yet oxygen interference remains a major challenge, significantly reducing accuracy at low concentrations. Here, we developed a universal enzymatic O2 scavenger composed of alcohol oxidase, catalase, and paraformaldehyde to eliminate O2 within the sensor by converting it to water. Unlike other oxidases, alcohol oxidase exclusively uses O₂ as an electron acceptor, preventing interference with the electron transfer chain involving the sensing oxidase. We demonstrated the compatibility of this O2 scavenger for calibration-free sensing of glucose, lactate and creatinine in the concentration range relevant to human health. Without the O₂ scavenger, sensor readings were less than 50% of those under inert gas conditions. With the O₂ scavenger accuracy improved to 99%, even at low substrate concentrations. The general compatibility of this alcohol oxidase-based O2 scavenger unlocks the full potential of oxidase-based biosensors for reliable, calibration-free point-of-use sensing.