In-vitro neuroprotective potential of Celastrol compound on SH-SY5Y neuroblastoma cells

Celastrol, a potent natural triterpene which acted as a neuroprotective agent and was isolated from Celastrus paniculatus Willd. roots. Neuroblastoma (SH-SY5Y) cells play very important role in the study and observation of the neuroprotective effect of the isolated compound Celastrol, neuroblastoma cells have been used to study and observe the neuroprotective effect of the isolated compound Celastrol. Rotenone a renowned neurotoxin which is used to induce (stimulate) the toxicity in SH-SY5Y cells in-vitro and further, the present study underseeks the neuroprotective effect of Celastrol on SH-SY5Ycells. Our results demonstrated that Celastrol confers protection against SH-SY5Y cells from rotenone-induced toxicity inhibiting Reactive Oxygen Species generation (ROS) using DCF-DA assay and also helps in retaining Mitochondrial Membrane Potential (ΔΨm) by using Rhodamine-123. Therefore, our study indicates that Celastrol has neuroprotective effects warranting further investigations into neuroprotective potential, which may be a possible therapeutic target in the treatment of neurodegenerative diseases.