The green synthesis of Zinc Oxide nanoparticles utilizing Camellia Sinensis var. Assamica found in commercial tea dust, and their broad spectrum antibacterial activity

10 October 2024, Version 2
This content is a preprint and has not undergone peer review at the time of posting.

Abstract

The antibacterial activity of biosynthesized Zinc Oxide (ZnO) nanofluid was investigated against three gram-positive bacteria (Enterococcus faecalis, Methicillin-resistant Staphylococcus aureus, Staphylococcus saprophyticus) and two gram-negative bacteria (Escherichia coli, Pseudomonas aeruginosa). Tea dust, obtained from “Lipton® black tea decaffeinated” tea bags, and distilled water was used to prepare an extract; with zinc acetate and sodium hydroxide as precursors to synthesize the ZnO nanoparticles, the extract was used as a reducing agent. ZnO nanoparticles were synthesized using a green method involving tea extract and characterized by EDX, TEM, and UV-VIS spectroscopy, revealing particles between 40–60 nm, with some particles as small as 18 nm, and the nanoparticles exhibited an agglomerated, sponge-like asymmetrical shape and maximum absorption at 380 nm. Despite its performance being slightly lower than standard antibiotics like Ciprofloxacin, Meropenem and Vancomycin, the ZnO nano-fluid still demonstrated significant antibacterial activity.

Keywords

Zinc Oxide
Nanoparticles
Biosynthesis
Antibacterial
Nanotechnology
Green Synthesis
Antibiotics

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