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Abstract
Cp*Co(III) complexes based on the ligand 1-amidino-2-thiourea (guanylylthiourea) have been synthesized and characterized. The antitumor potential of the synthesized complexes was evaluated in vitro using HeLa and HepG2 cell lines. The cytotoxicity of the complexes was assessed, along with their effect on the production of reactive oxygen species (ROS), activation of apoptotic pathways, and cell cycle progression. Finally, the acute toxicity of the complexes was evaluated using the model organism Caenorhabditis elegans.
Supplementary materials
Title
Synthesis and characterization of complexes and biological studies.
Description
Nuclear Magnetic Resonance (NMR).
Infrared Spectroscopy (IR).
High-Resolution Mass Spectrometry (HRMS).
Crystallographic Data of Single-crystals.
Biological Studies:
MTT Assays: The cytotoxicity of the Cp*Co(III) complexes was evaluated using MTT assays on HeLa and Hep G2 cell lines to determine cell viability.
ROS Activity: The production of reactive oxygen species (ROS) was measured to assess oxidative stress induced by the complexes in tumor cells.
Apoptosis Studies: Apoptotic pathways activated by the complexes were investigated, including the examination of key markers and mechanisms of apoptosis.
Flow Cytometry Studies: Flow cytometry was used to analyze cell cycle progression, apoptosis, and ROS levels, providing quantitative data on the cellular effects of the complexes.
Toxicity Studies in C. elegans: The acute toxicity of the Cp*Co(III) complexes was assessed using the model organism Caenorhabditis elegans, offering insights into their in vivo safety profile.
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