Controlling the Transmembrane Transport of Chloride by Dynamic Covalent Chemistry with Azines

Stimuli-responsive transmembrane ion transport has become a prominent area of research due to its fundamental importance in cellular processes and potential therapeutic applications. Commonly used stimuli include pH, light, and reduction or oxidation agents. This paper presents the use of dynamic covalent chemistry based on azine bonds to activate and modulate the transmembrane transport of chloride in liposomes. An active chloride transporter was obtained in situ within the lipid bilayer by dynamic azine metathesis. The transport activity was further tuned by changing the structure of the added azines, while the dynamic covalent chemistry could be activated by lowering the pH. This dynamic combinatorial chemistry approach holds great promise for drug delivery systems.