Efficient Parahydrogen Induced 13C Hyperpolarization on a Microfluidic Device

24 May 2024, Version 2
This content is a preprint and has not undergone peer review at the time of posting.

Abstract

We show the direct production and detection of 13C-hyperpolarized fumarate by parahydrogen-induced polarization (PHIP) in a microfluidic Lab-on-a-Chip (LoC) device and achieve 8.5% 13C polarization. This is the first demonstration of 13C-hyperpolarization of a metabolite by PHIP in a microfluidic device. LoC technology allows the culture of mammalian cells in a highly controlled environment, providing an important tool for the life sciences. In-situ preparation of hyperpolarized metabolites greatly enhances the ability to quantify metabolic processes in such systems by microfluidic NMR. PHIP of 1H nuclei has been successfully implemented in microfluidic systems, with mass sensitivities in the range of pmol √s. However, metabolic NMR requires high-yield production of hyperpolarized metabolites with longer spin life times than is possible with 1H. This can be achieved by transfer of the polarization onto 13C nuclei, which exhibit much longer T1 relaxation times. We report an improved microfluidic PHIP device, optimised using a finite element model, that enables the direct and efficient production of 13C hyperpolarized fumarate.

Keywords

NMR
hyperpolarization
microfluidics
lab-on-a-chip

Supplementary materials

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Title
Supporting Information: Efficient Parahydrogen Induced 13C Hyperpolarization on a Microfluidic Device
Description
Supporting document for manustcript titled "Efficient Parahydrogen Induced 13C Hyperpolarization on a Microfluidic Device". SI includes: 1. Multiphysics modelling. 2. Characterization of chip performance using hyperpolarized allyl acetate. 3. 1H NMR spectrum of [1 − 13C]fumarate. 4. 13C spectrum of 1M D-Glucose-1-13C. 5. Reference to raw data. 6 Technical drawings.
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