Preparation and biochemical evaluation of diallyl-thiosulfinate/polyoxyethylene conjugated pH-responsive micelle with enhanced stability, hydrosolubility and antibacterial properties

03 May 2024, Version 2
This content is a preprint and has not undergone peer review at the time of posting.


Diallyl-thiosulfinate (allicin) is a major bioactive chemical with several notable therapeutic characteristics of garlic (Allium sativum). Nonetheless, one of the biggest concerns regarding the extensive use of allicin in biopharmaceutical commodities is its unstable characteristics. Therefore, utilizing Polyoxyethylene (Brij S20 and Brij 58), appropriate pH-responsive micelle carrier systems have been designed to entrap and improve allicin's stability at an ambient temperature (25 °C) while preserving its quantity and biological activity. Comparing the Brij S20 with the Brij 58 micelle carrier system, the latter demonstrated superior stability and entrapment. In addition, it was found that allicin's stability in micellized condition is significantly influenced by both pH and temperature (p<0.05). Additionally, the liberation of allicin from micelle is greatly aided by acidic pH 1.5. The liberation of allicin from the micelle in a controlled manner using lower pH as stimuli may facilitate its biological action at an individual's gastrointestinal lumen or near cancer cell environment having lower pH. Additionally, it was made sure that the micellization method did not impair allicin's bioactivity or reduce appropriate biocompatibility. The current study increases the likelihood of creating a commercially available allicin-loaded, micelle-based formulation for application in biopharma and food related industries.


Controlled release
pH-responsive micelle

Supplementary materials

Supplementary Materials
ADMET analysis and predicted targets of allicin


Comments are not moderated before they are posted, but they can be removed by the site moderators if they are found to be in contravention of our Commenting Policy [opens in a new tab] - please read this policy before you post. Comments should be used for scholarly discussion of the content in question. You can find more information about how to use the commenting feature here [opens in a new tab] .
This site is protected by reCAPTCHA and the Google Privacy Policy [opens in a new tab] and Terms of Service [opens in a new tab] apply.