Mass spectrometry imaging with trapped ion mobility spectrometry (TIMS) facilitates spatially resolved chondroitin, dermatan and hyaluronan glycosaminoglycan (GAG) oligosaccharide analysis in situ.

22 April 2024, Version 1
This content is a preprint and has not undergone peer review at the time of posting.

Abstract

The spatial analysis of glycosaminoglycans (GAGs) by type and sulphation state is currently unobtainable. Here, we describe a mass spectrometry imaging (MSI) approach which enables the detection, identification, localisation and profiling of GAG oligosaccharides directly from retinal tissue. With in situ treatment of tissues with relevant chondroitinase enzymes, we liberate and spatially resolve chondroitin, dermatan and hyaluronan disaccharides through to hexasaccharides directly from tissue sections. We demonstrate, through the use of trapped ion mobility spectrometry (TIMS), that it is possible to both separate and semi-quantify isomeric GAG oligosaccharide ions across histologically relevant regions. Hence, we describe the first step towards the spatially resolved analysis of multiple GAGs and their oligosaccharide sulphation state(s) in tissues.

Keywords

glycosaminoglycan
mass spectrometry imaging
ion mobility spectrometry
glycomics
trapped ion mobility spectrometry

Supplementary materials

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Title
Supplementary data: Mass spectrometry imaging with trapped ion mobility spectrometry (TIMS) facilitates spatially resolved chondroitin, dermatan and hyaluronan glycosaminoglycan (GAG) oligosaccharide analysis in situ.
Description
Tables of ions and their putative and fragment identification. Confirmation of workflows on extra samples and all ion images studied.
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Comment number 1, Karen Campos: Jun 03, 2024, 05:22

Hello, this is amazing work! I noticed that the carbon count of the GAGs in Figure 1 is not accurate, the carbon on the carboxylic acid and the bent line on the structure account for the same carbon. I also noticed that there might be a slight typo in the caption of Figure 5, on the description of the H&E stain, it is referenced as H$E. I am looking forward to seeing this in print soon, best regards!