Boronic acid linked CPP for protein delivery

11 March 2024, Version 1
This content is a preprint and has not undergone peer review at the time of posting.


Studying functional protein delivery into live cells is important, ranging from fundamental research to therapeutics. Cell-penetrating peptides (CPPs) are known to deliver proteins with applauded efficacy and have gained importance for applications in protein therapeutics and exploring versatile cellular mechanisms. The primary aim of the work is to design a CPP as a tool/delivery vehicle for macromolecules, including proteins. In this work, boronic acid-linked cyclic deca arginine (cR10) is reported as an efficient CPP that exhibited threefold higher delivery of chemically synthesized ubiquitin (Ub) than pristine cR10-linked Ub, examined with live U2OS cells. As a futuristic plan, artificial intelligence machine learning-based rationale has been designed and proposed.


Cell Penetrating Peptide
Boronic Acid
Live Cells

Supplementary materials

SI of Boronic acid linked CPP for protein delivery
Details of synthetic procedures, experimental information, schemes, HPLCs, MS traces, and biological experimental details with future AI connection cartoon image.


Comments are not moderated before they are posted, but they can be removed by the site moderators if they are found to be in contravention of our Commenting Policy [opens in a new tab] - please read this policy before you post. Comments should be used for scholarly discussion of the content in question. You can find more information about how to use the commenting feature here [opens in a new tab] .
This site is protected by reCAPTCHA and the Google Privacy Policy [opens in a new tab] and Terms of Service [opens in a new tab] apply.