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Mass spectrometric evidence for the underrepresented RNA-protein crosslinks

04 March 2024, Version 1
Working Paper
This content is a preprint and has not undergone peer review at the time of posting.

Abstract

In protein-RNA complexes, UVC crosslinks proteins predominantly to uracil. Concrete evidence for other UVC-crosslinked RNA bases is lacking. Here, I elucidate mass spectrometric signatures of UVC-crosslinked guanine, cytidine, and methyladenine. This can help us capture the native protein-RNA interactions in more detail.

Keywords

RNA
RNA-binding protein
RNA-binding site
UVC-crosslink

Supplementary weblinks

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Github repository
Description
Fragpipe workflows, analysis results, protein sequence/annotation resources, and the custom Python codes used in this study have been deposited to the GitHub repository.
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Version History

Mar 04, 2024 Version 1

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The content is available under CC BY 4.0 [opens in a new tab]

DOI

10.26434/chemrxiv-2024-wzz3w
D O I: 10.26434/chemrxiv-2024-wzz3w [opens in a new tab]

Author’s competing interest statement

The author(s) have declared they have no conflict of interest with regard to this content

Ethics

The author(s) have declared ethics committee/IRB approval is not relevant to this content

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