Cambridge Open Engage home
What is Cambridge Open Engage?
How to Submit
Browse
About
News [opens in a new tab]

Single-benzene-based clickable fluorophores for in vitro and in vivo bioimaging

26 February 2024, Version 1
Working Paper
This content is a preprint and has not undergone peer review at the time of posting.

Abstract

A series of clickable single-benzene-based fluorophores derived from tetrafluoroterephthalonitrile (4F-2CN) is reported. Fluorophores based on a tetrahydroquinoxaline skeleton (2F-2CN-(β-NH2Ala)) exhibited improved photophysical properties owing to better planarity and conjugation over those with dihydro[1,4]thiazine skeleton (2F-2CN-Cys). These easily produced clickable fluorophores were successfully applied in in vitro and in vivo bioimaging after protein conjugation.

Keywords

Single-benzene-based fluorophore
Thiol-ene click reaction
Bioconjugation
Bioimaging
Actin filaments

Supplementary materials

Title
Description
Actions
Title
Supporting Information
Description
Experimental procedures and characterisation data for all new compounds, copies of UV/vis and fluorescence as well as 1H, 13C and 19F NMR spectra, XRD data for compound 9 and additional compounds, and experimental details for protein labelling studies and bioimaging.
Actions

Comments

Comments are not moderated before they are posted, but they can be removed by the site moderators if they are found to be in contravention of our Commenting Policy [opens in a new tab] - please read this policy before you post. Comments should be used for scholarly discussion of the content in question. You can find more information about how to use the commenting feature here [opens in a new tab] .
This site is protected by reCAPTCHA and the Google Privacy Policy [opens in a new tab] and Terms of Service [opens in a new tab] apply.

Version History

Feb 26, 2024 Version 1

Metrics

740
411
1
Views
Downloads

License

CC logo
CC
BY logo
BY
The content is available under CC BY 4.0 [opens in a new tab]

DOI

10.26434/chemrxiv-2024-s1fzw
D O I: 10.26434/chemrxiv-2024-s1fzw [opens in a new tab]

Funding

Wellcome Trust
101885/C/13/Z
Wellcome Trust
203276/B/16/Z
ERC-Actomyosin Ring
GA 671083

Author’s competing interest statement

The author(s) have declared they have no conflict of interest with regard to this content

Ethics

The author(s) declare that they have sought and gained approval from the relevant ethics committee/IRB for this research and its publication.

Share