Single-benzene-based clickable fluorophores for in vitro and in vivo bioimaging

26 February 2024, Version 1
This content is a preprint and has not undergone peer review at the time of posting.


A series of clickable single-benzene-based fluorophores derived from tetrafluoroterephthalonitrile (4F-2CN) is reported. Fluorophores based on a tetrahydroquinoxaline skeleton (2F-2CN-(β-NH2Ala)) exhibited improved photophysical properties owing to better planarity and conjugation over those with dihydro[1,4]thiazine skeleton (2F-2CN-Cys). These easily produced clickable fluorophores were successfully applied in in vitro and in vivo bioimaging after protein conjugation.


Single-benzene-based fluorophore
Thiol-ene click reaction
Actin filaments

Supplementary materials

Supporting Information
Experimental procedures and characterisation data for all new compounds, copies of UV/vis and fluorescence as well as 1H, 13C and 19F NMR spectra, XRD data for compound 9 and additional compounds, and experimental details for protein labelling studies and bioimaging.


Comments are not moderated before they are posted, but they can be removed by the site moderators if they are found to be in contravention of our Commenting Policy [opens in a new tab] - please read this policy before you post. Comments should be used for scholarly discussion of the content in question. You can find more information about how to use the commenting feature here [opens in a new tab] .
This site is protected by reCAPTCHA and the Google Privacy Policy [opens in a new tab] and Terms of Service [opens in a new tab] apply.