Recent advancement and applications of mass spectrometry imaging

Analysis concerning the spatial distribution of biomolecules in complex surfaces can be accomplished with mass spectrometry imaging [MSI]. The in situ direct determination of the abundance of lipids, peptides, proteins, and metabolites in segments of tissues enables MSI to be an effective technique. Molecule-specific visualization can be accomplished utilizing a variety of ionization techniques, each of which generates data with great spatial and mass accuracy and is suitable for a particular application. A chemical microscope that may be utilized for straightforward biomolecular assessment of histopathological tissue surfaces is generated through the integration of molecular classification with regional assessment. MSI effectively enables label-free identification and mapping of a variety of biological compounds, the presence or absence of which may be an obvious indication of disease pathogenesis. The MSI protocol's several phases must be carefully adhered to if one wants to detect the target analytes with the necessary spatial resolution. When combined with other imaging modalities, MSI can be used to ascertain the connection between changes in the spatial distribution of essential chemicals and other differences in the properties of the tissue. The findings of the examined empirical research illustrate how MSI represents a viable experimental approach with an assortment of applications. The paper will concentrate on technical developments, applications, challenges, and MSI's prospects moving forward.