Sub-millisecond Atomistic Simulations Reveal Hydrogen Bond-Driven Diffusion of a Guest Peptide in Protein-RNA Condensate

12 December 2023, Version 1
This content is a preprint and has not undergone peer review at the time of posting.

Abstract

Liquid-liquid phase separation mediated by proteins and/or nucleic acids is believed to underlie the formation of many distinct condensed phases, or membraneless organelles, within living cells. These condensates have been proposed to orchestrate a variety of important processes. Despite recent advances, the interactions that regulate the dynamics of molecules within a condensate remain poorly understood. We performed an accumulated 564.7μs all-atom Molecular Dynamics (MD) simulations (system size ~200K atoms) of model condensates formed by a scaffold RNA oligomer and a scaffold peptide rich in arginine (Arg). These model condensates contained one of three possible guest peptides: the scaffold peptide itself or a variant in which six Arg residues were replaced by lysine (Lys) or asymmetric dimethyl arginine (ADMA). We found that the Arg-rich peptide can form the largest number of hydrogen bonds and bind the strongest to the scaffold RNA in the condensate, relative to the Lys- and ADMA-rich peptides. Our MD simulations also showed that the Arg-rich peptide diffused more slowly in the condensate relative to the other two guest peptides, which is consistent with a recent fluorescence microscopy study. There was no significant increase in the number of cation-π interactions between the Arg-rich peptide and the scaffold RNA compared to the Lys-rich and ADMA-rich peptides. Our results indicate that hydrogen bonds between the peptides and the RNA backbone, rather than cation-π interactions, play the major role in regulating peptide diffusion in the condensate.

Keywords

Liquid-liquid phase separation
Protein-RNA Condensate
Molecular Dynamics Simulations

Supplementary materials

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Supporting Information
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SI figures
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SI Video 1
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Time-elapsed recruitment video of ADMA-rich peptide to pre-formed droplets.
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SI Video 2
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Time-elapsed recruitment video of Lys-rich peptide to pre-formed droplets.
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