Continuous Real-Time Detection of Serotonin using Aptamer-Based Electrochemical Biosensor

05 October 2023, Version 1
This content is a preprint and has not undergone peer review at the time of posting.

Abstract

Serotonin (5-HT) is a critical neurotransmitter involved in many neuronal functions, and 5-HT depletion has been linked to several mental diseases. The fast release and clearance of serotonin in the extracellular space, low analyte concentrations and a multitude of interfering species make detection of serotonin challenging. This work presents an electrochemical aptamer-based biosensing platform that can monitor 5-HT continuously with high sensitivity and selectivity. Our electrochemical sensor showed a response time of approximately 1 minute to a step change in the serotonin concentration (from 0 to 25 nM) in continuous monitoring using single frequency EIS (electrochemical impedance spectroscopy) technique. The developed sensing platform was able to detect 5-HT in the range 25 nM – 100 nM in the continuous sample fluid flow. The electrochemical sensor showed promising selectivity against other species with similar chemical structures and redox potentials including dopamine (DA), norepinephrine (NE), L-tryptophan (L-TP), 5-hydroxyindoleacetic acid (5-HIAA), and 5-hydroxytryptophan (5-HTP). The proposed sensing platform is able to achieve time resolution on the order of a minute with high selectivity in the nanomolar range demonstrating a potential for monitoring serotonin from neurons in organ-on-a-chip or brain-on-a-chip-based platforms.

Keywords

serotonin
real-time detection
neurotransmitters
electrochemical sensing

Supplementary materials

Title
Description
Actions
Title
Supplementary Information
Description
This supplementary information contains additional data on XPS, SPR, and EIS results for the manuscript "Continuous Real-Time Detection of Serotonin using Aptamer-Based Electrochemical Biosensor."
Actions

Comments

Comments are not moderated before they are posted, but they can be removed by the site moderators if they are found to be in contravention of our Commenting Policy [opens in a new tab] - please read this policy before you post. Comments should be used for scholarly discussion of the content in question. You can find more information about how to use the commenting feature here [opens in a new tab] .
This site is protected by reCAPTCHA and the Google Privacy Policy [opens in a new tab] and Terms of Service [opens in a new tab] apply.