Investigation of the time effect on the nanozyme-mediated oxidation of 3, 3’-diaminobenzidine by hydrogen peroxide

26 September 2023, Version 1
This content is a preprint and has not undergone peer review at the time of posting.

Abstract

Time effect on the nanozyme-mediated oxidation of 3, 3’-diaminobenzidine by hydrogen peroxide was investigated. Silver-based nanomaterials were synthesized and then used as peroxidase mimics. The time-dependent activity of the 3, 3’-diaminobenzidine oxidation catalyzed by silver-based nanomaterials was calculated by probing the color intensity of the produced brown-colored indamine polymer during the reaction at different time intervals. The time-dependent activity curve was used as an index for evaluating the time effect on the process, showed that the concentration of the brown-colored indamine polymer was increased by increasing the oxidation time and then leveled off, revealing saturation of active nodes of nanoparticles with nanozyme-substrate.

Keywords

silver nanoparticles
peroxidase-mediated oxidation
indamine polymer
time effect on oxidation
diaminobenzidine

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