Confocal Surface-enhanced Raman Imaging of the Intestine Barrier Crossing Behavior of Dual-functional Plasmonic Nanoplastics in Daphnia magna

12 September 2023, Version 2
This content is a preprint and has not undergone peer review at the time of posting.

Abstract

Due to wide spread, high concentrations, and easy bioavailability, nanoplastics (nPs) pose great ecological hazards both in the marine and freshwater ecosystems. To evaluate their impacts on the model water flea, Daphnia magna, and how they translocate from the intestine, the primary organ of accumulation, to the other body parts, is a key subject of research. In our current effort, we addressed the phenomenon of inter organ translocation of the nPs and suggested plausible mechanism of the process with the help of a dual functional plasmonic polystyrene (PS) nanoplastic (nPS) and confocal Raman mapping. We synthesized a ‘core-shell' polystyrene coated-nano gold particle and conjugated it with a Raman reporter, 4-mercapto benzoic acid (4-MBA). This dual functional plasmonic nanoplastic (model nPS) fulfills the purpose of nP as well as surface-enhanced Raman scattering (SERS) nano-probe for imaging. Upon exposure, the Daphnia showed uptake of the model nPSs mainly in the intestine tract. Exposure, beyond 4 h at concentration of 10 mg/L, exhibited inter organ translocation of the model nPSs to other parts in Daphnia body. Translocation was observed with the help of multilayer stack Raman mapping of the SERS signals coming from the model nPSs.

Keywords

translocation
intestine membrane
SERS
Raman mapping
Plasmonic nanoplastic
Daphnia magna

Supplementary materials

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Title
Confocal Surface-enhanced Raman Imaging of the Intestine Barrier Crossing Behavior of Dual-functional Plasmonic Nanoplastics in Daphnia magna
Description
We synthesized a polystyrene coated-nanogold ‘core-shell' particle (nPS@nAu), conjugated with a Raman reporter, 4-mercapto benzoic acid to the (4-MBA). This dual functional model nanoplastic (model nPS) fulfills the purpose of nP as well as surface-enhanced Raman scattering (SERS) nano-probe. Upon exposure, the Daphnia showed uptake of the model nPSs mainly in the intestine tract. Further exposure (beyond 4 h at model nPS concentration of 10 mg/L) exhibited inter organ migration of the model nPSs to other parts in the Daphnia body. Translocation of the model nPSs was observed with the help of multilayer stack Raman mapping of the SERS signals coming from the model nPSs.
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