Promiscuous yet specific: a methionine-aromatics interaction drives the reaction scope of the family 1 glycosyltransferase GmUGT88E3 from soybean.

13 July 2023, Version 1
This content is a preprint and has not undergone peer review at the time of posting.

Abstract

Family 1 glycosyltransferases (GT1s, UGTs) catalyze the regioselective glycosylation of natural products in a single step. We identified GmUGT88E3 as a particularly promising biocatalyst, able to produce a variety of pure, single glycosidic products from polyphenols with high chemical yields. We investigated this particularly desirable duality towards specificity, i.e., promiscuous towards acceptors while regiospecific. Using high-field NMR, kinetic characterization, molecular dynamics simulations and mutagenesis studies, we uncovered that the main molecular determinant of GmUGT88E3 specificity is a methionine-aromatic bridge, an interaction often present in protein structures but never reported for enzyme-substrate interactions.

Keywords

Glycosyltransferases
Polyphenols
NMR
Protein structure
Molecular dynamics

Supplementary materials

Title
Description
Actions
Title
SI for manuscript "Promiscuous yet specific: a methionine-aromatics interaction drives the reaction scope of the family 1 glycosyltransferase GmUGT88E3 from soybean"
Description
Michaelis-Menten plots, HPLC chromatograms, NMR data, Molecular dynamics analysis, Experimental section
Actions

Comments

Comments are not moderated before they are posted, but they can be removed by the site moderators if they are found to be in contravention of our Commenting Policy [opens in a new tab] - please read this policy before you post. Comments should be used for scholarly discussion of the content in question. You can find more information about how to use the commenting feature here [opens in a new tab] .
This site is protected by reCAPTCHA and the Google Privacy Policy [opens in a new tab] and Terms of Service [opens in a new tab] apply.