The semisynthesis of nucleolar human selenoprotein H

16 June 2023, Version 1
This content is a preprint and has not undergone peer review at the time of posting.


The human selenoprotein H is the only selenocysteine-containing protein that is located in the cell’s nucleolus. In vivo studies have suggested that it plays some role in DNA binding, consumption of reactive oxygen species, and may serve as a safeguard against cancers. However, the protein has never been isolated and, as a result, not yet fully characterized. Here, we used a semi-synthetic approach to obtain the full selenoprotein H with a S43T mutation. Using biolayer interferometry, we also show that the Cys-containing mutant of selenoprotein H is capable of binding DNA with sub-micromolar affinity.


chemical protein synthesis
nucleolar protein
human selenoprotein H


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