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High Throughput Metabolomics using 96-plex Isotope Tagging

31 May 2023, Version 1
Working Paper
This content is a preprint and has not undergone peer review at the time of posting.

Abstract

Liquid Chromatography-mass spectrometry (LC-MS) based metabolomics suffers from extended duty cycles and matrix-dependent quantitation. Chemical tags with 96 unique masses are reported which alleviate the metabolomic workflow bottleneck and allow for absolute quantitation. A metabolic screen for carboxylic acids was performed on mammalian cells deprived of various nutrients and showed 24% RSD and analysis of 288 samples in 2 hours.

Keywords

Multiplexing
Metabolomics
Isotopes
High-throughput screening

Supplementary materials

Title
Description
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Title
Analyte m/z list
Description
This matrix details the expected m/z for each isotope variant of every analyte investigated.
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Title
Tag isotope purity matrix
Description
Raw data detailing all sites of isotope overlap. Tags are numbered in order of decreasing mass.
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Title
Cell media table
Description
Details the media components used for culture. Nutrients in red were added manually or excluded to create the deprived media stocks.
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Title
Tag plate maps
Description
Details which tags were used for each biological experiment.
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Tag characterization
Description
HRMS and NMR characterization of the 96 synthesized tags.
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Supporting information
Description
Contains supplemental figures, tables, and methods referenced in the manuscript.
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Version History

May 31, 2023 Version 1

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The content is available under CC BY NC ND 4.0 [opens in a new tab]

DOI

10.26434/chemrxiv-2023-zrjth
D O I: 10.26434/chemrxiv-2023-zrjth [opens in a new tab]

Funding

National Institutes of Health
5R01GM134081

Author’s competing interest statement

The author(s) have declared they have no conflict of interest with regard to this content

Ethics

The author(s) declare that they have sought and gained approval from the relevant ethics committee/IRB for this research and its publication.

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