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Abstract
A new bioink consisted of single-walled carbon nanotubes (SWCNTs) conjugated with anti-HBsAg antibodies (SWCNT-Ab) was applied for the detection of HBsAg. The electrical conductivity of the printed film was optimized by printing multiple layers of the SWCNT-Ab bioink. The biosensors showed a linear response range from 1-10 mM HBsAg, and the response began to plateau after 10 mM, with a detection limit of 0.5 mM. The inkjet printed biosensors showed stable resistance and a linear response to increasing concentrations of HBsAg. The study suggests that this method has the potential for producing low-cost and sensitive biosensors for the early detection and accurate diagnosis of HBV in resource-limited settings.
