Charged Chiral Derivatization for Enantioselective Imaging of D-, L-2-hydroxyglutaric acid Using Ion Mobility Spectrometry/Mass Spectrometry

17 April 2023, Version 1
This content is a preprint and has not undergone peer review at the time of posting.

Abstract

A newly synthesized charged chiral tag enabled enantioselective imaging of D-, L-2-hydroxyglutaric acid, which are independently associated with the regulation of DNA methylation. The tag-conjugated diastereomers were ionized efficiently through MALDI, separated by ion mobility spectrometry, and further separated from other molecules in mass spectrometry. On-tissue chiral derivatization using the tag facilitated the visualization of different distributions of the two isomers in the mouse testis.

Keywords

Chiral
Imaging
Ion mobility spectrometry
Mass spectrometry
Derivatization
2-Hydroxyglutaric acid

Supplementary materials

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Electronic Supplementary Information
Description
experimental methods, supplemental figures, spectroscopic data.
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Graphical abstract
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Graphical abstract
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