Synthesis of polydiacetylene (PDA) particles for detection of histamine

30 March 2023, Version 1
This content is a preprint and has not undergone peer review at the time of posting.

Abstract

Histamine poisoning as a problem of public health and safety attracted attention worldwide. There is a significant need for devices that are capable of measuring histamine levels rapidly with high sensitivity. In this study, a colorimetric sensor based on polydiacetylene (PDA) was developed for histamine detection. Under optimal conditions, the biosensor showed a linear response to different concentrations of histamine within the range of 21 ppm to 210 ppm with a low detection limit of 10.1 ppm. Moreover, the biosensor was successfully employed to detect the histamine level in the spiked canned fish sample. With high sensitivity and recovery values, this assay can be used to monitor the quality of canned fish, suggesting that it holds great potential for on-site detection and real-time monitoring.

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